Animal Cell Technology: Basic & Applied Aspects: Proceedings by Shinji Iijima (Editor), Ken-ichi Nishijima (Editor)

By Shinji Iijima (Editor), Ken-ichi Nishijima (Editor)

Entire updates of swiftly increasing fields of animal telephone expertise Covers all issues from educational to commercial concerns

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Extra info for Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 17th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), ... Cell Technology: Basic & Applied Aspects)

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One of the most important functions of the human intestines is as a barrier against foreign substances like bacteria and chemicals. In this intestinal immune system, immue cells such as macrophages located in the intestinal epithelium interact with epithelial cells via soluble factors. Any disorder of this interaction causes defective overproduction of some inflammatory cytokines and subsequent mucosal inflammation; for example, IBDs such as Crohn’s disease (CD) and ulcerative colitis (UC). Several function of human intestinal epithelial cells have been reported, including the absorption of nutrients and the barrier function against xenobiotics like pathogenic bacteria or chemical substances.

The frozen tissues were ground in the frozen state, and homogenized tissue powders were used for non-targeted FTMS analysis. A total of 68 samples (duplicate analysis of control diet SHPsp liver and plasma, male and female n = 4; duplicate analysis of broccoli fed SHPsp liver and plasma, n = 4 male, n = 5 female) were processed via a proprietary extraction method optimized to separate the metabolites into multiple extracts based upon their polarity and acid/base chemistry as shown in Figure 1. Extracts were analyzed by direct injection Fourier Transform Ion Cyclotron Resonance mass spectrometry (FT-ICR-MS).

Biotech. Biochem. 67(6): 1312-1318. Son D-O, Satsu H, Kiso Y, and Shimizu M (2004) Characterization of the carnosine uptake and its physiological function in human intestinal epithelial Caco-2 cells. Biofactors, 21(1-4): 395-398. Stallmach A, Giese T, Schmidt C, Ludwig B, Mueller-Molaian I, Meuer SC (2004) Cytokine/chemokine transcript profiles reflect mucosal inflammation in Crohn's disease. Int J Colorectal Dis. 19(4): 308-315. , 3-6-6 Asahi-machi, Machidashi, Tokyo 194-8533, Japan Abstract: To generate industrially applicable new host cell lines for antibody production with optimizing antibody-dependent cellular cytotoxicity (ADCC) we focused on the most important carbohydrate structure “fucose residues attached to the innermost GlcNAc residue of N-linked oligosaccharides via Į-1,6 linkage” (Shields, 2002; Shinkawa, 2003), and succeeded in disrupting both FUT8 (Į-1,6-fucosyltransferase gene) alleles in Chinese hamster ovary (CHO) cell line by sequential homologous recombination.

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