Bacterial and Bacteriophage Genetics: An Introduction by Edward A. Birge

By Edward A. Birge

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For the case in which there are only two outcomes, successes and failures, probability can be rigorously defined as the lim _s_ n~oo S +f where n is the total number of trials, s is the number of different ways to succeed, and f is the number of different ways to fail. 36 2: The Laws of Probability and Their Application to Prokaryote Cultures The terms permutation and combination refer to sampies which contain more than one item. If the order of the items within the sampie is considered, then the sampie is a specific permutation.

B. Transduction In transduction, a bacterial virus (bacteriophage or phage) is intimately involved in the genetic transfer. Phage infections begin with the adsorption of the phage particles to specific receptor sites on the surface of the cello The nucleic acid contained inside the protein coat is then transferred into the cytoplasm of the bacterial cell, where it becomes metabolically active and undergoes the processes of replication and transcription. Typically, there are two possible outcomes to a phage infection.

How many phage-infected cells would you expect to find in a sampie? What is the probability of having no phage-infected cells in the sampie? How should the sampie size be adjusted so that the probability of no phage-infected cells becomes 5%? Aseries of sampies has been taken from a bacterial culture, and it has been observed that 67% of the sampies have no mutant cells in them and 27% of the sampies have exactly one mutant cello What percentage of the sampies would you expect to have two mutant cells?

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