By Jan Michiels, Maarten Fauvart
This quantity provides a finished number of equipment which were instrumental to the present figuring out of bacterial persisters. Chapters within the ebook hide issues starting from basic equipment for measuring persister degrees in Escherichia coli cultures, protocols for the selection of the persister subpopulation in Candida albicans, quantitative measurements of style I and kind II persisters utilizing ScanLag, to in vitro and in vivo models for the learn of the intracellular task of antibiotics. Written within the hugely winning Methods in Molecular Biology series structure, chapters comprise introductions to their respective issues, lists of the required fabrics and reagents, step by step, conveniently reproducible laboratory protocols, and tips about troubleshooting and averting recognized pitfalls.
Authoritative and state-of-the-art, Bacterial patience: equipment and Protocols brings jointly the main revered researchers in bacterial patience whose reports will stay very important to realizing this box for a few years to come.
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Additional resources for Bacterial Persistence: Methods and Protocols
ChpBK cleave translated RNAs and are counteracted by tmRNA. J Mol Biol 332 (4):809–819 39. Christensen SK, Gerdes K (2004) Delayedrelaxed response explained by hyperactivation of RelE. Mol Microbiol 53(2):587–597 40. Gonzalez Barrios AF, Zuo R, Hashimoto Y, Yang L, Bentley WE, Wood TK (2006) Autoinducer 2 controls biofilm formation in Escherichia coli through a novel motility quorumsensing regulator (MqsR, B3022). J Bacteriol 188(1):305–316 41. Yamaguchi Y, Inouye M (2011) Regulation of growth and death in Escherichia coli by toxinantitoxin systems.
2. 5 % (w/v). Pour powder on the top of water in a beaker, stir to dissolve the soluble components, bring to boil in a microwave oven, and boil for 1 min to dissolve agar. Transfer by 200 mL into 250 mL flasks; autoclave at 121 C for 15 min. Allow to cool to ~55 C and pour plates. Pour ~20 mL of LB agar per 10 cm polystyrene Petri dish (see Note 3). 3. 45 g Na2HPO4 (see Note 4) in 800 mL water, and fill up to 1000 mL. Dilute 10Â in water to prepare 1Â PBS. Autoclave and store at room temperature.
1128/JB. 01231-09 7. Spoering AL, Lewis K (2001) Biofilms and planktonic cells of Pseudomonas aeruginosa have similar resistance to killing by antimicrobials. J Bacteriol 183(23):6746–6751. doi:10. 2001 8. Keren I, Minami S, Rubin E, Lewis K (2011) Characterization and transcriptome analysis of Mycobacterium tuberculosis persisters. MBio 2 (3):e00100–e00111. 1128/mBio. 00100-11 9. Slattery A, Victorsen AH, Brown A, Hillman K, Phillips GJ (2013) Isolation of highly persistent mutants of Salmonella enterica serovar Typhimurium reveals a new toxin-antitoxin module.