Chromosome Painting: Principles, Strategies and Scope by Arun Kumar Sharma, Archana Sharma

By Arun Kumar Sharma, Archana Sharma

Chromosome portray is the main sleek and novel strategy for at once opting for a number of gene sequences at the same time within the chromosome, by using particular probes in molecular hybridization. Its answer levels from unmarried replica to whole genome sequences. it's now utilized in plant, animal, and human platforms, in gene mapping, id of genetic problems, evolutionary reports, and gene move experiments. This treatise is the 1st of its style to hide the strategy with all its changes and purposes. it's designed for normal use through postgraduate scholars and learn staff in cellphone and molecular genetics, plant and animal sciences, agriculture, drugs, and phylogenetic studies.

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Additional info for Chromosome Painting: Principles, Strategies and Scope

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1994 Glas et al. 1999b Chowdh ary & Raud sepp 2000b Comparative chromosome painting or Zoo-FISH Comparati ve chromoso me painting, also commonl y referred to as cross species chromosome paint ing or Zoo-FISH, came to limelight during early 90s'. , [46, 105]). As a result, comparati ve chromosome painting data for all human chromosomes are avail able for several primate species. The finding s have been instrumental in delineating homologies between their chrom osomal compliments 41 [22, 47 , 48, 59, 83, 95, 104, 106].

We can tentati vel y speculate that increased numbers of tandem telomeric repeats are induced by the growth of constituti ve heterochromatin at the same site; if true, the small FITC signals repre sent areas that have not yet undergone growth of heterochromatin (amplification). The C-band and telomeric repeat heteromorphisms of gorilla chromosome 18 provide strong support for this postulated mechanism of chromosomal change. (b) Area s of C-banded heterochromatin stained with DAPI , but not labeled by the telomere primer during PRINS, are probably comprised of mostly AT-rich sequence, and do not harbor telomeric repeats.

L'\'ej 3. / 8 70 Frederiks llerg C. Denm ark Abstract. A mo ng th e adva nced karyo ty pe a na lys is ap proac hes e m braced by a nima l cy toge ne tics duri ng th e pa st decade. ch ronioso tuc IJlIintin g ha s had th e g reates t impact. Ge ne ratio n o f c hro moso me s pec ific paint s is co nsi dere d pi vot al to hi s de vel opm en t. Additio na lly, abi lity to use these pain ts across sp ec ies (referre d to as Zoo-FIS H or co mparative p a intin g ) is undi sputedl y th e most imp ort ant breakth rou gh that has contributed to o ur ability to compar e karyotype s of a wide ran ge of evo lut io narily hi ghl y di ver ged species.

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