Cytometric Analysis of Cell Phenotype and Function by Desmond A. McCarthy, Marion G. Macey

By Desmond A. McCarthy, Marion G. Macey

Movement cytometry and laser scanning cytometry are more and more utilized in scientific and examine settings as a result of advancements in tool layout and computing strength and the elevated availability of fluorescent brokers. This booklet offers a complete creation to the speculation and scientific purposes of those suggestions within the overview of mobile phenotype and serve as. With an emphasis on scientific relevance, the publication offers the rules and capability of cytometry within the research of phenomena together with cell-mediated cytotoxicity, metabolic burst, phagocytosis, cell-cell aggregation, receptor laying off, and apoptosis. the amount publications the reader via info interpretation, quality controls systems, pitfalls, and difficulties.

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Fig. 17 Bone marrow aspirate from a 48-year-old woman with chronic myeloid leukaemia in blast crisis. The CD45+ cells which were gated (A) and subsequently displayed as three separate Xuorescein isothiocyanate (FITC) versus phycoerythrin (PE) histograms (B). See text for full details.

Cells are stained with Wright–Giemsa and B-cells are shown in the top row and T-cells in the bottom. Fig. 15 Relocalisation of cells for in situ hybridisation by epiXuorescence microscopy. The top row is the B-cells and the bottom row is the T-cells. Fig. 16 Enhancement of the epiXuorescence video images of the Xuorescence in situ hybridisation probe spots in Fig. 15. Fig. 17 Bone marrow aspirate from a 48-year-old woman with chronic myeloid leukaemia in blast crisis. The CD45+ cells which were gated (A) and subsequently displayed as three separate Xuorescein isothiocyanate (FITC) versus phycoerythrin (PE) histograms (B).

12 Peripheral blood from a patient with chronic lymphocytic leukaemia. Fig. 13 Selection of cells for relocalisation from the sample illustrated in Fig. 12. Fig. 14 Light microscopy of the cells relocated in Fig. 13. Cells are stained with Wright–Giemsa and B-cells are shown in the top row and T-cells in the bottom. Fig. 15 Relocalisation of cells for in situ hybridisation by epiXuorescence microscopy. The top row is the B-cells and the bottom row is the T-cells. Fig. 16 Enhancement of the epiXuorescence video images of the Xuorescence in situ hybridisation probe spots in Fig.

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