By John R. Masters (Editor), Bernhard O. Palsson (Editor), James A. Thomson (Editor)
If you want to develop or signify embryonic stem cells or convince them to distinguish right into a specific mobilephone variety, then this publication comprises info that's important for your good fortune. the purpose is to supply transparent basic directions and protocols for becoming, preserving and characterizing embryonic stem cells and information of some of the tools used to make stem cells differentiate into particular telephone kinds. The contents should be of curiosity to stem mobilephone biologists, tissue engineers and scientists wishing to take advantage of embryonic stem cells for healing reasons. each one bankruptcy has been written and edited by way of across the world revered scientists operating on the leading edge of technological advancements in human embryonic stem cells.
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Extra info for Embryonic Stem Cells (Human Cell Culture)
F. (2002) Biochemical properties of a keratan sulphate/chondroitin sulphate proteoglycan expressed in primate pluripotent stem cells. J. , 200: 259–265. A. (2004) Derivation of embryonic stem-cell lines from human blastocysts. N. Engl. J. , 350: 1353–1356. E. (2005) Efficient differentiation of human embryonic stem cells to definitive endoderm. Nat. , 23: 1534–1541. W. (2004) Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells. Nat. , 22: 53–54. , et al. (2005) Cellular differentiation hierarchies in normal and culture-adapted human embryonic stem cells.
2007 Springer. qxd 18/4/07 4:44 PM Page 28 Laslett et al. 28 antigen expression provides a simple means for detecting abnormal hESC even at low frequencies in mixed cultures by flow cytometry. The true frequency of development of karyotypic or other genetic abnormalities in hESC culture is not known, but it is almost certainly a function of culture methodology and passage level. Karyotypic abnormalities that persist in culture are often accompanied by changes in cellular growth, differentiation, and survival, rendering interpretation of any data on affected cell lines problematic.
Many of the mouse-based techniques have proven amenable to translation to human ES cell systems, with varied levels of success. Here, we provide an overview of the recent strategies and successes in the genetic manipulation of human ES cells. R. ), Embryonic Stem Cells, 41–52. © 2007 Springer. qxd 18/4/07 4:45 PM Page 42 42 Zwaka 2. EXOGENOUS GENE EXPRESSION IN HUMAN ES CELLS Genes have been introduced into human ES cells by both transfection and infection techniques. Transient transfection, the simplest method for introducing new genes into various cell types, may be achieved using liposomes and other cationic/ lipid-based particles, brief electric shocks (electroporation), or other strategies.