Evolving Genes and Proteins. A Symposium Held at the by Vernon Bryson, Henry J. Vogel

By Vernon Bryson, Henry J. Vogel

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Additional info for Evolving Genes and Proteins. A Symposium Held at the Institute of Microbiology of Rutgers: the State University with Support from the National Science Foundation

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DR. VOGEL: In the paper I believe you have in mind [ J . Marmur, M. E . Cahoon, Y. Shimura, and H. J . Vogel, Nature, 197, 1228 ( 1 9 6 3 ) ] , it was suggested that the third peak corresponds to DNA from specialized homogeneous structures or from a virus. It was, in fact, our working hypothesis that the third peak represents kinetoplast DNA. The possibility of a virus, though not considered likely, would have been consistent with the narrow width of the band involved. However, even if no virus is found, we have a set of remarkable ecological relationships: at their base is a milk weed with which the Oncopeltus bug is specifically associated; the bug is the host for the protozoon Crithidia which, in turn, harbors its endosymbiotic bacterium.

I wouldn't doubt it, but we will see in the future, maybe in the next symposium. DR. HOROWITZ: I wonder if I could ask Ruth Sager a question. How much DNA is there per chloroplast in Chlamydornonas? DR. SAGER: Our best estimate of the amount of chloroplast DNA comes from isolating total cell DNA and then fractionating it according to buoyant density in a cesium chloride density equilibrium gradient. W e have found about 5 % of the DNA in a satellite band associated with the chloroplasts. The DNA obtained from isolated chloroplasts is greatly enriched for this fraction, but of course there are losses in preparation, and the final values of DNA per chloroplast by this method are about 1% of the cell DNA.

Discussion of Part I Κ. V. ΤΗΙΜΑΝΝ, Ν . Η . HOROWITZ, Ε . L . TATUM, L . SPETNER, Η . J . VOGEL, R. SAGER, W. BRAUN, Ο. Ε . LANDMAN, S. W. Fox, K. BLOCH, Ν . H. G u T T M A N , P . H. ABELSON, C. B . VAN N I E L , C. PRICE, C. PONNAMPERUMA CHAIRMAN ΤΗΙΜΑΝΝ: The papers presented are now open for discussion. DR. HOROWITZ: I would like to ask Dr. Tatum two questions: First of all, I wasn't sure whether your cytoplasmic slow grower, which Wilson has used for injection experiments, was poky or not? The second question is, do you know how much DNA is present per mitochondrion in Neurospora?

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